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This class provides functions that do alignments.

src/b/i/bioa-1.24/bioa/io/amplicon.py   bioa(Download)
              ("H", "T"): 2, ("V", "A"): 2, ("V", "C"): 2, ("V", "G"): 2,
              ("V", "T"): -1, ("T", "T"): 2}
    from Bio.pairwise2 import align
    # wrapper function to find best local alignment
    def _best(reference, seq):