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SetMinimumPaneSize(self, int min)

Sets the minimum pane size in pixels.

The default minimum pane size is zero, which means that either pane
can be reduced to zero by dragging the sash, thus removing one of the
panes. To prevent this behaviour (and veto out-of-range sash
dragging), set a minimum size, for example 20 pixels. If the
wx.SP_PERMIT_UNSPLIT style is used when a splitter window is created,
the window may be unsplit even if minimum size is non-zero.

src/w/x/wx_py-   wx_py(Download)
        self._shouldsplit = True
        self.Bind(wx.EVT_SIZE, self.SplitterOnSize)